Factors involved in promoting caulogenesis from hypocotyl explants ofPhyllanthus fraternuswere studied. Hypocotyl explants were cultured on B5medium supplemented with 2,4-D or NAA in the presence and absence of BAP (at concentrations 0, 10−7, 10−6and 10−5M). Adventitious shoots differentiated from callus developed from the cut ends of 12.5 of the hypocotyl segments cultured on medium supplemented with 10−6M BAP in combination with 10−6M 2,4-D or 10−6M NAA. Profuse rooting occurred from the hypocotyl explants on medium supplemented with 10−6M BAP + 10−6M NAA. Incorporation of casein hydrolysate in B5medium along with 10−6M BAP + 10−7M 2,4-D enhanced the frequency of cultures with adventitious shoots upto 68.0. Glutamine, glutamic acid or proline could partially substitute for the effect of casein hydrolysate. Amongst the hypocotyls from 3–14 d old seedlings, the best caulogenesis was obtained with hypocotyls from 7 d old seedlings both in presence or absence of casein hydrolysate. Best rooting of shoots was achieved on half-strength B5medium supplemented with 10−6M IBA. After hardening, plantlets were successfully
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