Gel permeation chromatography using controlled‐porosity glass. I. Polyacrylamide–formamide solution

摘要

AbstractThe process of characterizing polyacrylamide and its partially hydrolyzed materials by gel permeation chromatography was examined. The use of controlled‐porosity glass and formamide as the stationary phase and the eluent, respectively, resulted in chromatographic behavior in accord with the hydrodynamic volume concept for polyacrylamide fractions. The addition of a salt (KCl) to the eluent was found to retard the elution of the hydrolyzed polyacrylamid