Design of camp–CRP‐activated promoters inEscherichia coli

摘要

SummaryWe have studied thedoeP2promoter ofEscherichia colito define features that are required for optimal activation by the complex of adenosine 3′, 5′ monophosphate (cAMP) and the cAMP receptor protein (CRP). Systematic mutagenesis ofdeoP2shows that the distance between the CRP site and the ‐10 hexamer is the crucial factor in determining whether the promoter is activated by camp–CRP. Based on these observations, we propose that camp–CRP‐activated promoters can be created by correctly aligning a CRP target and a ‐ 10 hexamer. This idea has been successfully tested by converting both a CRP‐in‐dependent promoter and a sequence resembling the consensus ‐10 hexamer to strongly camp–CR