AbstractThree cultivars of wheat showing different levels of spontaneous karyotypic instability were studied regarding their stabilityin vitrofor a number of characters, i.e. chromosome structure, gliadin pattern, glume and grain colour, awn type, chlorophyll pigmentation and plant morphology. The progenies of somaclones derived from immature embryos of both aneuploid and euploid plants were used in this study along with foundation seeds and a large number of their sexual progeny in order to discriminate between pre‐existent variability and any novel variation induced by thein vitroculture. Only one translocation not described before and a new gliadin pattern were detected which could be ascribed to the effects of tissue culture, suggesting that this technique is not effective for inducing novel variation for breeding purposes in whea
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