Surface properties and flocculations of globulin‐immobilized fine polymer particles

摘要

AbstractCharacteristics of globulin‐immobilized styrene/acrylamide/acrylic acid copolymer poly(St/AAm/AA) fine particles and their flocculation by an antigen–antibody reaction were investigated as a function of acrylamide (AAm) content. Immobilization of globulin was carried out through octamethylenediamine with a carbodiimide reaction. The increase in AAm content in particles decreases the amounts of bovine γ‐globulin (BγG) immobilized, shifts the isoelectric points of BγG‐immobilized poly(St/AAm/AA) particles to acidic pH, and increases the dispersion stability of BγG‐immobilized poly(St/AAm/AA) particles. The flocculation rates of antihuman epidermal growth factor (hEGF) immunoglobulin (IgG)‐immobilized poly(St/AAm/AA) fine particles of low AAm content by the antigen‐antibody reaction are higher than that of anti‐hEGF IgG‐adsorbed polystyrene particles, whereas those of high AAm content are lower and decreased with increasing AAm content. © 199