AbstractThree commercial triaryl phosphates, Pliabrac 521, Reofos 95 and Reolub hyd 46, were tested for acute toxicity against the brackish‐water harpacticoidNitocra spinipesBoeck (Crustacea) and the zebra fish,Brachydanio rerioHamilton‐Buchanan. The 96‐h LC50 values ranged from 0.27 to 1.75 mg/L, with Pliabrac 521 as the most toxic and Reolub hyd 46 as the least toxic. The zebra fish was more than 10 times less sensitive to the tested compounds than wasN. spinipes.Low to moderate accumulation of triaryl phosphates was observed. An accumulation factor (AF) of 100 to 2,000 was observed for Pliabrac 521 from water to bleak,Alburnus alburnusL. For the various triaryl phosphates, a dietary accumulation of 0.06 to 0.6 from food to minnow,Phoxinus phoxinusL., was observed. The most hydrophilic compounds were readily cleared from fish when the fish were placed in clean water, whereas the most lipophilic ones were still detectable, albeit in low concentrations, in the fish after a 2‐week depuration period. A rotatory‐flow behavioral study did not reveal any effects on swimming performance in the bleak, but reproduction was affected as a result of long‐term (163 d) exposure of minnows to subacute concentrations of P
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