Canine GM1GangliosidosisAn Ultrastructural and Biochemical Study

摘要

The ultrastructural and biochemical features of canine GM1gangliosidosis were studied. beta;-Galactosidase activity assayed using both skin fibroblast tissue culture strains and fresh skin revealed enzyme activities in three groups (normals, heterozygotes, and homozygotes) corresponding to an autosomal recessive inheritance. The concentration of ganglioside GM1was greatly increased in cerebral gray matter and kidney. A striking elevation of tissue oligosaccharides was found in liver, kidney, and spleen. Most neurons in the cerebral cortex and deep gray matter were filled by spherical lamellated inclusions. Hepatocytes contained vacuoles with an amorphous granular material which may correspond to the accumulation of galactoseoligosaccharides determined chemically. The disease in dogs has features similar to both the infantile and juvenile form of human GM, gangliosidosis.