The inactivation of various aminoglycosides prior to sterility testing has been studied. The preparation of the enzyme used in the inactivation, a 3‐N‐acetyl transferase of wide substrate specificity fromEscherichia coliJR 225, is described. The enzyme is partially purified and some of its kinetic parameters described. The extent of the inactivation of the individual aminoglycosides can be related to the physiological efficiency of the enzyme. The lyophilization of the components of the inactivating system is successful when undertaken individually, but not toget
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