Summary.The bacterial flora of the film which developed on experimental trickling filters irrigated with domestic sewage was dominated byAcinetobacterand yellow‐pigmented Gram negative rod‐shaped organisms and it was deduced that purification in certain aerobic waste treatment processes may depend upon an association of these 2 organisms. Further evaluation of the operational procedures likely to have a selective influence on the microbial association which becomes dominant was made in studies using synthetic sew
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