The reduction to the micro-scale of a recently described electrophoretic method for the evaluation of GABA catabolism by GABA-T is presented. The micromethod involves the electrophoresis, in 1 mm diam. capillaries, of small samples of mixtures of 14CGABA and its metabolites. By coupling this procedure to previously devised micromethods, it was possible to evaluate GABA-T attack to14C labeled GABA diffusing across a single microdissected neuronal membrane.
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