Intestinal dimensions of mice divergently selected for body weight

摘要

abstract_textpBackground: Selection for body weight provides important animal models for studying mechanisms of growth regulation, This study evaluated growth responses of the gastrointestinal tract (GIT) to long-term selection of mice for high (H line) or low (L line) 8-week body weight as compared with random-bred controls (C line),/ppMethods: Weights and dimensions of the various parts of the GIT were recorded from 8-month-old mice, For light microscopic stereological analyses, samples were taken from eight equidistant locations covering the whole jejunum/ileum. Vertical sections were used for estimation of fractional volumes of mucosa, submucosa, and muscularis and of villous surface area density and for measurement of villus length,/ppResults: Differences between groups in weights and dimensions of the various parts of the GIT were more pronounced in the proximal than in the distal segments, with greatest values in H, followed by C and L mice, Relative to body weight, intestinal growth was similar in the three lines, except for significantly (P 0.001) increased relative weights of jejunum/ileum, caecum, and colon in L mice, The fractional volume of mucosa and villus length decreased, whereas the fractional volumes of submucosa and muscularis increased from the proximal to the distal locations, The absolute volume of mucosa was greatest in H mice, followed by C and L mice, Relative to body weight, the volume of mucosa was significantly (P 0.01) greater in L mice than in the two other lines, The mean total villous surface area of jejunum/ileum was significantly (P 0.01) different among the three lines (L line: 144 cm(2) C line: 227 cm(2): H line: 304 cm(2)) but proportionate to differences in metabolic body weight,/ppConclusions: Selection for body weight affected various parts of the GIT to a different extent, The parameters investigated changed markedly along the small intestine, demonstrating the need for systematic sampling, Vertical section stereology provides unbiased estimates of total villous surface area, which is a parameter of major biological significance. (C) 1998 Wiley-Liss, Inc./p/abstract_text