Acetylcholinesterase (AChE) activity in the rat subfornical organ at 8, 12, 18, 24, 26, 28 and 30 h postmortem was evaluated by the Koelle and Friedenwald method. AChE activity was unchanged in animals 12 h postmortem. When the postmortem period was extended, enzymatic activity diminished, disappearing almost completely after 30 h.
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