A note on the use of a plasmid as a DNA probe in the detection of a Lactobacillus fermentum strain in porcine stomach contents

摘要

A plasmid (about 50 kb) was used as a DNA probe to enumerate, by colony hybridization, a strain ofLactobacillus fermentumin the stomach contents of eight piglets. The population sizes obtained by colony hybridization were in agreement with estimated levels calculated on the basis of plasmid profiling of colonies isolated at random from the total lactobacillus population.