Studies of the effects of ethylene on yeast alcohol dehydrogenase activity

摘要

The temperature at which incubation with ethylene takes place has a significant effect on the purified alcohol dehydrogenase (ADH) activity subsequently determined at room temperature. Ethylene can be separated completely from ADH on a Sephadex column. Factors, such as the ionic strength of the buffer and the presence of gelatin and NAD during the incubation with ethylene can modify the effects of the gas. In yeast cells the effects of ethylene on ADH activity are similar to those in the purified system. The presence of cyloheximide in the incubation medium did not suppress the effects of ethylene on ADH activity. Ethylene may induce its effect, directly, through involvement in hydrophobic bonding in enzymes.