Untersuchungen über die lichtabhängige Carotinoidsynthese

摘要

In conidia-free submerged cultures ofNeurospora crassathe various steps of light-dependend carotenoid synthesis were studied. The mycelium produces small amounts of pigments even in the dark. The data obtained are in part in good agreement with earlier results ofZalokarand show that the light-induced pigment production starts after a lag-period of 40 min and is finished after 6–8 hours; the photoreaction is saturated by relatively small dosages. In contrast toZalokar'sresults we found that for photoinduction the reciprocity law holds true. The photoreaction is saturated by a certain amount of light independently of the light-intensity. Actidion (Cycloheximide) inhibits carotenoid synthesis completely when added before or up to 10 min after the onset of illumination, whereas addition 60 min after illumination already has no effect. Comparison with the results obtained withFusariumshows that the reaction mechanism is very similar in both organisms, though the various steps seem to proceed faster inNeurospor