Recruitment of effector lymphocytes by initiator lymphocytes: role of a trypsin‐sensitive membrane component

摘要

AbstractIn earlier studies we found that initiator T lymphocytes sensitizedin vitroagainst fibroblasts were able to recruit immunospecific effector T lymphocytesin vivoin syngeneic mice. In the present study, to investigate the possible function in effector cell recruitmentof sensitizing antigens passively adsorbed onto the initiator cell membrane, we treated the initiator lymphocytes with trypsin. We found that trypsin inhibited recruitment. However, the initiator cells spontaneously recovered their recruiting ability after incubationin vitrofor 4 h in the absence of contact with sensitizing antigens. Recovery could be blocked by cycloheximide. Hence, it appears that recruitment depends on the intrinsic metabolic function of the initiator lymphocytes and is not due merely to passively‐adsorbed antigen carried over into the recipient mous