Characterization and Quantification of Anti‐T in Human Serum Using a Reliable Hemolysis Test

摘要

Abstract.The need of fresh‐frozen donor plasma with a low level of anti‐T has been emphasized recently. Anti‐T, as administered by transfusion of fresh‐frozen plasma, has been accused repeatedly of enhancing hemolysis in septic children with T transformation of red cells. Therefore, a new hemolysis test for the quantification of anti‐T in human serum has been developed. With our test, anti‐T‐poor plasma donors can be found. Additional results raise substantial doubt as to the pathogenetic role of anti‐T in the development of the hemolytic‐uremic syndrome, found in septic children with red‐cell T transformation. It is impossible to predict in vivo hemolysis induced by anti‐T knowing the temperature characteristics and the ionic conditions causing this antibody to mediate hemolysis in vitro. Obviously, T transformation itself plays the major pathogenetic role in these patients, and not the presence of anti‐T. In the case of disseminated intravascular coagulation, a content of anti‐T cannot be construed as prohibiting transfusion of fresh‐fro