Essais de caracteacute;risation cytochimique des structures de l'interface au niveau du reacute;seau de Hartig dans l'association ectomycorhizienne entre la truffe (Tuber melanosporum) et le noisetier (Corylus avellana)

摘要

The interface established betweenTuber melanosporumandCorylus avellanawas studied cytochemically (PATAg test, Swift's reaction, wheat germ agglutinin ndash; colloidal gold labelling) to characterize cell wall and matrix components. By combining ultrastructural cytochemistry and selective extractions of polysaccharides by various solvents (EDTA, dimethyl sulfoxide, methylamine) or enzymes (pectinase, cellulase, cytohelicase), some ultrastructural features were made evident. Ultrastructural cytochemical tests demonstrate different domains in the matrix. Cell wall and matrix components are similar, but the fungal chitin is not detected in the matrix.Key words: ectomycorrhiza, interface, ultrastructural cytochemistry, selective extractions.