Laboratory culture of gametophytic stages of the marine macroalgaechampia parvula(rhodophyta) andlaminaria saccharina(phaeophyta)

摘要

AbstractMethods to help characterize the toxicity of effluent discharges within the National Pollutant Discharge Elimination System have been developed for the marine red algaChampia parvulaand are currently being developed for the brown algaLaminaria saccharina. These methods require maintaining male and female gametophytes in laboratory cultures. Aseptic techniques are recommended even though the cultures are not intended to be axenic; the purpose is to guard against microalgal contamination.C. parvulacan be grown at room temperature, butL. saccharinarequires a controlled temperature chamber (12–15°C). Both species can be cultured successfully in either natural or artificial seawat