Characterization and functional properties of a novel monoclonal antibody which identifies a T cell receptor in chickens

摘要

AbstractA monoclonal antibody, mAb6, was produced that specifically bound to chicken T lymphocytes. Immunofluorescence analysis using a fluorescence‐activated cell sorter revealed that the antibody reacted with approximately 50 of blood lymphocytes and with approximately 40 of splenocytes and thymocytes. It did not react with bursa1 cells and erythrocytes. Among different types of hemopoietic cell lines, only a T cell line was reactive with mAb6. When coupled to Sepharose 4B beads, mAb6 was found to be highly mitogenic for chicken T cells. In soluble form, mAb6 inhibited concanavalin A‐induced T cell proliferation and the cytotoxic activity of allosensitized T cells, the inhibition occurring in a dose‐dependent manner for both assays. Thus, the tissue distribution and the effects of this antibody on T cell responses suggest that mAb6 recognizes a T cell receptor present on a large proportion of chicken T