The Influence of Combined Treatment of Fast Neutrons and NEU on the Mutation Spectrum in Peas

摘要

AbstractInvestigations were carried out on mutation induction in peas by using fast neutrons (Nf), N‐nitroso‐N‐ethylurea (NEU), And a combined treatment of both agents. In total, 1314 mutants were isolated representing a wide mutation spectrum. In comparison 10 the single mutagens the combined treatment acted as a different, third if it were mutagen, being the most effective and producing the highest number and the widest spectrum of induced mutants. Nf was clearly least effective with regard to the number of mutants, but it did induce the widest mutation spectrum per 100 induced mutants. Moreover, some mutation types appeared only with a given mutagen and in a given genotype. The greatest number of such specific mutants was selected in the line Wt 3527 after the combined treatment; among them some new genes inPisumwere found, e.g.orange pod, necrosis, arthriticordeterminate g