An isocratic high-performance liquid chromatography method for the assay of plasma amrinone is described. Plasma amrinone is extracted using protein precipitation with an internal standard, separated with a reverse phase column, and detected using ultraviolet absorption. Each run is completed within 10 min. The assay can detect amrinone concentrations between 0.5 and 10.0 mu;g/ml, within the accepted therapeutic range. The assay has a within-day coefficient of variation of
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