On Line Detection of Plasma Borne Vasoconstrictor by the Use of High Pressure Liquid Chromatography

摘要

An on line detection system for a plasma borne vasoconstrictor was developed using a rat heart bioassay and a reversed-phase high pressure liquid chromatograph (HPLC). Partially purified plasma borne vasoconstrictor, which is yet to be characterized, was fractionated by HPLC, and the output from the unit was introduced into the rat heart bioassay system directly. The on line HPLC-rat heart bioassay system detected the active fraction from approximately 20 substances in the partially purified plasma. This system enabled rapid and reproducible identification of the active vasoconstrictor in plasma.