Clonal micropropagation was optimized for Roman chamomile,Anthemis nobilisL. (Compositae), through shoot tip culture. Apical and axillary buds produced multiple shoots in Murashige and Skoog's (MS) basal medium supplemented with BA (0.5 mg/liter) and IAA (0.5 mg/liter). A positive effect of 0.1 percent yeast extract on shoot multiplication and growth was detected. Roots were induced on shoots using an MS-medium without BA. Rooting was stimulated by the addition of 0.1 mg/liter of IBA or IAA to the culture media. Thein vitromultiplication gave rise to healthy plantlets that were successfully established in soil and had normal development and morphology.
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机译：对罗马洋甘菊（Anthemis nobilisL）的克隆微繁殖进行了优化。（菊科），通过枝尖培养。顶芽和腋芽在补充有BA（0.5 mg/L）和IAA（0.5 mg/L）的Murashige和Skoog（MS）基底培养基中产生多个芽。检测到0.1%酵母提取物对枝条繁殖和生长有积极影响。使用不含 BA 的 MS 培养基在枝条上诱导根系。通过向培养基中加入 0.1 mg/L 的 IBA 或 IAA 来刺激生根。体外繁殖产生了健康的植株，这些幼苗成功地在土壤中建立，并具有正常的发育和形态。
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