Inhibition ofCandidaadhesion to buccal epithelial cells by an aqueous extract ofAllium sativum(garlic)

摘要

The effect of pre‐incubation of eitherCandidaor buccal epithelial cells (BEC) with different concentrations of aqueous garlic extract (AGE) was investigated, as well as the effect of mouth rinse with AGE on the adhesion of yeast to BEC. Adhesion ofCandidaspp. to BEC was significantly reduced after both short and long time exposure of yeast to AGE. A similar inhibition of adherence was observed upon pre‐incubation of BEC with AGE. The adherence‐inhibition activity of AGE treatment was antagonized by thiols such as l‐cysteine, glutathione and 2‐mercaptoethanol. In addition, germ‐tube formation was suppressed whenC. albicanscells were pretreated with AGE. There was a significant reduction in the adherence of yeasts to BEC collected immmediately or 15 min after an oral rinse with AGE. No statistical significance in the adhesion of BEC collected 30 min after oral rinse with AGE and control BEC was observed. The diminished adherence ofC. albicansto BEC after exposure to various concentrations of garlic may have clinica