The effects of H$and fusicoccin (FC) on stomatal opening in the dark were investigated using epidermal strips ofCommelina communisandVicia fabacv. Ryosai Issun. Citrate-phosphate buffer induced maximal opening of stomata at pH 3.0 when tested over the range of 2.7 to 5.0. HCl at 1 mM also induced stomatal opening without appreciable accumulation of K$in the guard cells. After 4 hr treatment with 10μM FC, stomata opened with concomitant accumulation of K$in the guard cells, although 1–2 hr treatment caused opening without concomitant K$increase.These results suggest that stomatal opening can be caused by salt accumulation and/or changes of the physicochemical conditions in the cell wall of the guard cells due to high acidi
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