Transformation of Diploid and Tetraploid Potato Clones with therol CGene ofAgrobacterium rhizogenesand Characterization of Transgenic Plants

摘要

AbstractDiploid and tetraploid potato clones were transformed with therolC gene fromAgrobacterium rhizogenesunder the control of the cauliflower‐35‐S promoter. Parameters proper to the sink and source attributes and hormone treatments have been considered to study the effect of the gene on yield physiology of the potato plant. The expression of therol Cgene in diploid and tetraploid potato lines induced drastic developmental abnormalities like dwarfism and increased tillering. Hormonal treatments revealed a different behavior ofrolC transformams and controls. This supports the conclusion that the gene product of the ml C gene interacts with the hormonal status of the plant. Grafting experiments showed the non‐ditfusable nature ofrol Cgene product. Tuberization was studiedin‐vitroandin‐planta.Relevant differences in tuber shape and eye number were noted betweenrol Ctransfonnams and controls. Tuber yieldsin‐plantain greenhouse or in growth chamber resulted higher in the controls; a significant increased number of tubers per plant was found, however, in both diploid and tetraploidrol Ctransformants. The effect of therolC gene was apparently too drastic for diploid and tetraploid potatoes; weaker and tissue specific promoters may be necessary to induce only those modifications which are wanted for this crop. The experiments nevertheless proved that one single gene was capable in potato to affect substantially the manifestation of vieldmg