Simultaneous Determination of Allantoin and Creatinine in Urine by a Rapid Reversed-Phase Liquid-Chromatographic Method

摘要

A simple, rapid, reproducible RP-HFLC method for a simultaneous analysis of allantoin and creatinine in sheep urine, is described. Separation was achieved on a Novapak-C18 (3.9#xD7;300 mm) column by isocratic elution. Quantitation and detection limits were determined. Detection was effected at 218 nm. Retention times of allantoin and creatinine were 4.4 and 5. 8 min, respectively.