Growth regulators,Rhizobiumand nodulation in peas

摘要

Indole-3-acetic acid (IAA) has been identified in the culture medium of nodulating and non-nodulating strains ofRhizobium lebuminosarumby gas chromatography-mass spectrometry. The levels of IAA produced by the different strains have been quantified using multiple ion monitoring and a deuterated internal standard. Indole-3-acetic acid is produced in the absence of exogenous tryptophan in all strains but its level is greatly stimulated by applied tryptophan. No correlation has been established between the ability to nodulate peas and the ability to produce IAA.