Abstract:TrJ14 is a cytotoxic human IgG1Λ hybridoma mAb that recognized a novel HLA‐A epitope expressed by lymphoblastoid B cells that are homo‐ or heterozygous for A2, A3, A11, A30, A31, A33, A68 and A69. Based on these results, the HLA type of cell line TEM (10w9057) was retyped as A66. When peripheral blood T cells isolated freshly from 265 HLA‐typed normal individuals served as targets, TrJ14 killed cells expressing two TrJ14‐positive HLA‐A alleles, as well as the majority of cells having one TrJ14‐positive and one TrJ14‐negative HLA‐A antigen. However, TrJ14 failed to recognize or reacted weakly with most HLA‐A2 and ‐A3 heterozygous normal T cells when A2 or A3 was coexpressed together with a TrJ14‐negative antigen. The serological reactivity of TrJ14 correlated with the amino acid valine and aspartic acid at positions 76 and 77 of the αl‐domain helix. These amino acids were shared exclusively by all the
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