Adventitious Shoot Organogenesis and Plant Regeneration from Leaf and Cotyledon Explants of Citruttus colocynthis

摘要

Plant regeneration protocol for Citrullus colocynthis L. was achieved from leaf and cotyledon explants. Explants were cultured on MS medium supplemented with 2,4-D, in combination with BAP, TDZ, Kn and Zeatin. Leaf explants are the best source for theinduction of high percentage of caulogenesis and regeneration (45) on MS + 2.0 mg.Lr' Kn + 1.0 mg.IT1 TDZ. In cotyledon derived callus cultures 2,4-D + Zeatin combination played an important role in induction of multiple shoots (15-18) with 50 of regeneration response. Each cotyledon explants produce an average number of shoots (9.75 +- 0.2) with average shoot length 3.66 ±0.1.