Isolation of Pinus radiata genomic DNA suitable for RAPD analysis.

摘要

A protocol is presented for Pinus radiata genomic DNA isolation based on an alkyltrimethyl-ammonium bromide (CTAB) method described for other woody species. The method involves mortar grinding of tissue, a modified CTAB extraction employing high salt concentrations and polyvinyl pyrrolidone, a RNase A ribonuclease treatment and successive isoamyl alcohol-chloroform extractions. The yield was approximately 15 鎔 DNA per 100 mg of initial fresh plant material. The genomic DNA obtained by this method was suitable for use in simple sequence repeat and RAPD reactions. This extraction method would allow the molecular analysis of shoots from different clones within P. radiata families. This full text of this paper is available on-line through the WorldWide Web at http://www.chemweb.com/ library/kluwer/pmbrdisplay.exe?jcode=pmbr.