Efficient iin-vitro/i regeneration from mature leaf explants of iScoparia dulcis/i L., an ethnomedicinal plant.

摘要

A plant regeneration protocol via multiple-shoot induction using leaf explants from field-grown mature plants of Scoparia dulcis L. (Scrophulariaceae), an ethnomedicinal plant, was established. Explants were cultured on Murashige and Skoog (MS) medium with different concentrations of 6-benzylaminopurine (BAP, 13.2, 17.6 and 22.2 micro M) in combination with indole-3-acetic acid (IAA, 0.5 and 2.8 micro M) or napthalene-3-acetic acid (NAA, 0.5 and 2.6 micro M). The maximum number of shoots (14.0+or-1.14) with longest shoot length (2.97+or-0.18) were obtained directly (without an intervening callus phase) from the leaf explants using a combination of BAP (22.2 micro M) and IAA (0.5 micro M). BAP used in combination with NAA produced fewer shoots per explant as compared with the use of IAA and the regeneration was mediated by callus formation. For root induction, the elongated shoots were separated and transferred onto MS medium supplemented with indole-3-butyric acid (IBA, 4.9 micro M). Rooted plantlets (90) were successfully transferred to soil in the greenhouse with a 75 survival rate.