Cloning of a two-component signal transduction system ofXanthomonas campestrispv.phaseolivar.fuscansstrain BXPF65

摘要

A putative two-component signal transduction system was amplified and cloned from the plant pathogenic bacteriumXanthomonas campestrispv.phaseolivar.fuscansisolate BXPF65. The 620 bp amplified fragment was sequenced and analyzed with the BLAST Enhanced Alignment Utility (BEAUTY). BEAUTY analysis indicates that the putative xanthomonad response regulator contains regions which matched to an ATP phosphorylation site, a #x3C3;54-factor interaction domain, and the #x201C;receiver#x201D; domains of various transcriptional regulators of the two-component signal transduction systems. Similar analysis indicates that the putative histidine kinase has homology with conserved #x201C;transmitter#x201D; domains of sensor proteins in two-component signal transduction systems. RFLP analysis using the putative signal transduction system showed polymorphisms among the strains.