The fluorescence of solutions of fluorescein and acridone has been studied as a function of (1) the concentration of iodide added with a constant concentration of the fluorescer and (2) the concentration of the fluorescent substance. It is shown that the results are fitted much better by the equation of Stern and Volmer than by that of Perrin. In the absence of a quencher the intensity of the fluorescence observed is given by a function of the concentration which takes into account the following three effects: (1) the absorption of the exciting light by the solution, (2) the absorption of the fluorescent light, and (3) the quenching by the molecules of the fluorescent substance. No other effects needed to be considered for the substances studied.
展开▼
