A new, simple and sensitive method for the determination of diiodohydroxyquin in dosage forms using high-performance liquid chromatography (HPLC) has been developed. Diiodohydroxyquin is extracted with isopropanol, the solution is evaporated to dryness, the residue is reconstituted in methanol containing 0.01 phenyl salicylate (internal standard) and injected. A plot of peak height ratio (diiodohydroxyquin/internal standard) vs. concentration of diiodohydroxyquin gave a straight line. The column used was a commercially available #x3BC; Bondapak C18cartridge 10 cm #xD7; 8 mm, and the mobile phase was a methanol: 0.05 M orthophosphoric acid (75:25) mixture at a flow rat of 3 cm3per minute. Retention times for diiodohydroxyquin and phenyl salicylate were 3.85 min. and 3.0 min. respectively.
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