The Use of Large Volume Injections for the Isocratic Separation of Phenylthiohydantoin Amino Acids by Microbore Liquid Chromatography

摘要

The use of microbore columns in high performance liquid chromatography (HPLC) has remained limited primarily due to difficulties in adapting conventional HPLC analysis to the smaller volumes and lower flow rates of microbore chromatography while maintaining high quality chromatographic results.