Determination of susceptibility ofStaphylococcus aureusto methicillin by luciferim‐luciferase assay of bacterial adenosine triphosphate

摘要

McWalter, P.W. 1984. Determination of susceptibility ofStaphylococcus aureusto methicillin by luciferin‐luciferase assay of bacterial adenosine triphosphate.Journal of Applied Bacteriology,56, 145–150.Susceptibility of 50 strains ofStaphylococcus aureusto methicillin was determined by disc diffusion, minimum inhibitory concentration (MIC) test and luciferin‐luciferase assay of bacterial adenosine triphosphate (ATP). ATP concentration time curves calculated on eight strains (four sensitive, four resistant) incubated at 30d̀C indicated 2.5 h as the optimum time for determination of methicillin susceptibility. The ATP concentration and relative light unit (RLU) value of each test broth (antibiotic‐containing), expressed as a percentage of its own control broth (antibiotic‐free) indicated values of<30 for ATP and<40 for RLU to be indicative of methicillin sensitivity. Single time point ATP assays carried out on the 50 strains after 2.5 h at 30d̀C correlated exactly with disc diffusion and MIC. Luciferin‐luciferase assay of bacterial ATP appeared to be a reliable, rapid technique for determining the susceptibility ofStaph. aureust