Characterization of an antibiotic produced by Alteromonas luteoviolacea Gauthier 1982,85 isolated from Kinko Bay, Japan

摘要

An antibiotic produced byAlteromonas luteoviolaceastrain 9K‐V10 was recovered after cold acetone precipitation of culture supernatant fluids or lysates that had beenfrozen and thawed. The precipitate obtained from cell‐free lysates was fractionated by DEAE ion‐exchange chromatography. Further purification by gel‐filtration chromatography yielded a single peak of antibiotic activity that corresponded to a protein peak with a molecular mass of approximately 100 kDa. After non‐denaturing polyacrylamide gel electrophoresis, antibiotic activity co‐migrated with a protein band. The isoelectric point of the antibiotic was estimated to be 7·7. Treatment of the concentrated active fraction with proteinase K or heating at 70°C for 10 min resulted in total loss of antibiotic activity. These results show that the antibiotic produced byAlt. luteoviolacea9K‐V10 is of a prote