Oligoepoxide-based monoliths: Synthesis and application as affinity capillary column for enrichment of immunoglobulin G

摘要

A series of new epoxide-based monoliths is synthesized by self-polymerization of polyglycerol polyglycidyl ether as porous support for affinity chromatography. Porosity and mean pore sizes are investigated as a function of the porogen ratio and analyzed by mercury porosimetry. The morphologies are visualized by environmental scanning electron microscope. With a particularly rigid monolith prepared with 75 vol porogen (66 porosity and 12 μm mean pore size), a capillary column (0.5 mm ID) is prepared and directly functionalized with recombinant protein A to extract immunoglobulin G from rabbit serum.