The distribution of the (TTAGGG)n telomeric sequence was studied in chromosomes of the wood lemming, Myopus schisticolor, by fluorescence in-situ hybridization. As expected, the hybridization signals were observed at telomeres of all chromosomes. However, quite a number of interstitial telomeric sites were present in the pericentric heterochromatic regions. Consistent strong hybridization signals were also seen at one terminus of chromosomes 5, 7 and 12--15. By post-hybridization G-banding and silver-staining, the large blocks of the telomeric sequences on chromosomes 5 and 12 were localized to nucleolus organizer regions (NORs).
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机译:通过荧光原位杂交研究了(TTAGGG)n端粒序列在木旅鼠Myopus schisticolor染色体中的分布。正如预期的那样,在所有染色体的端粒上观察到杂交信号。然而,在中心周围异色区存在相当多的间质端粒位点。在5号、7号和12--15号染色体的一个末端也观察到一致的强杂交信号。通过杂交后 G 条带和银染,将 5 号和 12 号染色体上的端粒序列的大块定位于核仁组织者区 (NOR)。
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