Phenotypic and Cellular Changes Associated with Loss of Antigen-Induced Function in Epstein-Barr Virus-Specific Human T-Cell Hybrids

摘要

Human T-cell hybrids were constructed from the HAT-sensitive human T-cell line ‘JMAE’ and an uncloned population of T cells from donor SW (SW-T; partner cell) known to have strong specificity for the autologous Epstein-Barr virus (EBV)-transformed B cell, SWEBV. The resulting hybrids, 1A9, 1D12 and 2C8 were shown to have similar specificity and to increase their rate of proliferation in response to SWEBV. Furthermore, the hybrids responded to other suitable EBV-transformed targets, in an major histocompatibility complex-restricted manner. Continued study of these cells revealed that they were functionally, phenotypically and chromosomally unstable. The present report details the loss of specific function and the associated changes in cell-surface markers, chromosome number and basal proliferation r