Reversed Phase High Performance Liquid Chromatographic Studies for Homologous Series of Polyunsaturated Fatty Acids on a Commercial #x3BC;Bondapak Free Fatty Acid Column

摘要

Reversed phase high performance liquid chromatography (RPHPLC) has been proven to be one of the most efficient and reproducible separation methods for fatty acids since 1974. Particularly, in the case of homologous series studies, RPHPLC is an exclusively unavoidable method1. The thermodynamic and kinetic studies for free fatty acids, oleic acid (octadecenoic acid 18:l#x394;9c), lioleic acid (octadecadienoic acid, 18:2#x394;9c, 12c), linolelaidic acid (octadecadienoic acid, 18:2#x394;9t, 12t), linolenic acid (octadecatriecoic acid, 18:3#x394;9c, 12c, 15c), #x3B3;-linolenic acid (octadecatrienoic acid, l8:3#x394;6c, 9c. 12c), eicosadienoic acid (eicosadienoic acid, 20:2#x394;11c, 14c) and eicosatrienoic acid (eicosatrienoic acid, 20:3#x394;11c, 14c, 17c) were performed on a 10#x3BC; commercial alkyl phenyl free fatty acid column. The mobile phase was acetonitrile, water and tetrahydrofuran in the ratio of 45:35:20 v/v. The isocratic elution method was adopted in these studies using differential refractometer as detector. The retention data was obtained and thereby capacity factors, enthalpies of adsorption were calculated. Finally the retention mechanism was explained.