Twenty‐seven strains ofClostridium botulinumtype E were inoculated into six culture media and examined for production of spores. Thirty‐six similar media were then inoculated with each of five strains. Strains varied greatly in their ability to form spores in a given medium. Spore formation varied markedly with casein digests and peptones of different manufacturers. Some differences were broadly indicated between strains from the Pacific and from Scandina
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