Herpes simplex virus type 1 (HSV-1) strains vary widely with regard to neurovirulence, but their tropism for specific central nervous system structures and their ability to induce seizures are poorly defined. We have used the clonally related plus;GC and -GC strains of HSV-1 to define the pathophysiological basis of neurovirulence in a rabbit model. Following intranasal inoculation, plus;GC infection was nearly uniformly fatal while -GC infection was asymptomatic. The plus;GC infected animals developed electroencephalographic (EEG) abnormalities which preceded severe motor seizures. Tropism of the plus;GC strain for specific CNS nerve centers and the expression of viral antigens within them correlated with its virulence. Although both viruses invaded and replicated within the brain, plus;GC replicated to slightly higher titers and expressed more abundant viral antigen than -GC. The relatively less efficient replication of mdash; GC appeared to correlate with its temperature-sensitive phenotype in vitro. Both plus;GC and -GC antigens were found in cerebral cortical layers IV-VI, and in several central nervous system trigeminal and olfactory system structures. However, plus;GC spread more completely throughout the brain to involve the amygdala, nucleus accumbens, several brainstem nuclei and the locus ceruleus. The plus;GC antigens were also found in cerebral cortical layer I of animals that developed seizures. These results indicate that the ability of HSV-1 to induce electrophysiologic brain abnormalities is associated with its ability to replicate within specific brain nerve centers.
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