Interleukin-2 Responses of MRL/lpr Mouse Splenocytes and Lymph Node Cells Induced by TPA and A23187

摘要

Treatment of splenocytes and lymph node cells of 5 month-old MRL/lpr mice with TPA induced IL-2-dependent proliferation of the cells in the presence of CA++. The induced response was inhibited completely by a monoclonal antibody to IL-2 receptor. The combination of TPA and A23187 in the Ipr cells induced both proliferation and production of IL-2 in a Ca++ – dependent fashion. The proliferative response of the Ipr cells was equivalent to that of congenic (MRL/+/+) or normal cells, but the quantity of IL-2 secreted from the Ipr cells was significantly less than that of the controls. Actinomycin D, but not mitomycin C, blocked IL-2 secretion from the treated Ipr cells indicating de novo synthesis of IL-2 mRNA in the cells. Thus the Ipr lymphocytes can be activated to proliferate in response to IL-2, yet they do not secrete IL-2 at a normal level even if activation signals are transmitted into the cell