Determination of inosine-5′-monophosphate in the presence of inosine and hypoxanthine with an enzyme sensor

摘要

A sensor for the determination of inosine-5′-monophosphate (IMP) in the presence of inosine and hypoxanthine was prepared by a combination of the enzyme system shown below and an oxygen electrode.$$begin{gathered} IMPxrightarrow{{5' - nucleotidase}}{text{inosine}}xrightarrow{Pi}{{{text{nucleoside phosphorylase}}}} hfill {text{hypoxanthine}}xrightarrow{{text{O}}_{text{2}} }{{{text{xanthine oxidase}}}}{text{uric acid}} hfill end{gathered}