Influence of a negatively charged surface (Teflon disk) onEntomophaga aulicaeprotoplast morphogenesis under mass fermentation conditions

摘要

The effects of a negatively charged surface (Teflon disk) on protoplast morphogenesis for the fungusEntomophaga aulicaeunder mass fermentation conditions were determined. The control consisted of a vessel lacking such a disk. In the presence of the disk the initial three and sequentially produced protoplast stages (spindle shaped, early fusion sphere, and late fusion sphere protoplasts) recycled with the early fusion sphere predominating. The production of the subsequent and walled stage (i.e., hyphal body) was suppressed. The results are in contrast with those obtained in a previous study using a neutral (Mylar) and a positively charged (polypropylene) disk in which hyphal body production was enhanced. This technique provides a new and subtle approach for altering protoplast developmental patterns which avoids the use of mutagens or added chemical metabolic inhibitors.Key words:Entomophaga aulicae, fungal protoplast morphogenesis, negatively charged surface, mass fermentation.