Schnelldiagnose von #x201C;Kohlen-monoxidvergiftungen#x201D; und Quantitative Bestimmung von CO-Hb in Blut

摘要

A quick diagnosis of carbon monoxide poisoning is a very difficult clinical problem. This paper describes a photometric method which permits a reliable diagnosis in a time of 7 to 10 minutes. Only 0.3 ml whole blood are necessary for the total procedure. Carboxyhemoglobin changes in absorption maximum slightly after reduction, whereas Oxyhemoglobin shows a significant change in extinction. Measurement of the hemolysate at the same wave length before and after alkaline reduction results in large differences in the extinctions (very large for-Oxyhemoglobin and very small for Carboxyhemoglobin). Calibration curves are prepared by calibrating rest-extinction defined as: Extinction (578 nm) after reduction/Extinction (578 nm) before reduction. 100