An inverted repeat preceding theBacillus subtilis glpDgene is a conditional terminator of transcription

摘要

SummaryTheBacillus subtilis glpDgene, encoding glycerol‐3‐phosphate (G3P) dehydrogenase, is preceded by a promoter and an inverted repeat which is located between the promoter and the glpD coding region. The Inverted repeat acts as a transcriptional terminatorin vitro.Expression ofglpDis induced by G3P in the presence of theglpDgene product. Full‐lengthglpDtranscripts can be detected only in glycerol‐induced cells. The majorglpDtranscript is initiated from theglpDpromoter but minor amounts of larger transcripts, possibly initiated at upstreamglppromoters, can also be found. In uninduced cells short transcripts are present, corresponding to initiation at theglpDpromoter and termination at the inverted repeat. Upon induction, these short transcripts disappear and are replaced by full‐lengthglpDtranscripts. The 3′‐ends of full‐lengthglpDtranscripts were mapped to an Inverted repeat located immediately downstream ofglpD.These results show thatglpDofB. subtilisis regulated by termination/antitermination o